Biological mass spectrometry

• Introduction of biophysical MS methods at Roche (with and by A.Topp): Affinity selection mass spectrometry (ASMS) and native mass spectrometry of soluble and membrane proteins
• Establishment of in-house metabolic biomarker facility to supply target engagement data for discovery biology using LC/MS, GC/MS and NMR (with V. Stierlin, I. Ruf, M. Wittig, A. Stämpfli, C. Tournoux, A.Topp, B.Bürgi)
• Re-establishment of NMR and LC/MS based metabolomics capability for biomarker discovery in (stem) cell cultures (A. Stämpfli, A. Ross) to discover metabolism reactions to genomic change and markers for phenotypic screening
• Introduction of MS readouts for bioassays at Roche Basel –  automation and introduction of a LC/MS based drug-drug interaction assay from Roche Nutley, assays for enzyme inhibitors for cerebrosides, steroids, lipids, (with I. Ruf) multienzyme profiling readout for the optimization of an ultraselective steroid synthesis inhibitor (with M. Wittig)
• Development of stable isotope tracer applications e.g. for the determination of biosynthesis rates of cholesterol or glucose (with V. Stierlin, C. Albrecht, M. Wittig)

Chemistry support

• Introduction of LC/MS compound quality control hand-in-hand with narrow bore NMR and complete informatics workflow including structure display and structure based automatic ESI-MS and UV signal prediction (with C.Albrecht)

DMPK/ADME

• Establishment of rapid metabolite identification service for discovery projects (I. Ruf)
• Introduction of LC/MS rodent DMPK analysis for infectious disease discovery, including cassette dosing experiments (C. Albrecht, V. Stierlin, I. Ruf)

Informatics

• IT: Development and introduction of several LIMS systems, automated data analysis systems for LC/MS bioanalysis and compound quality control with integration of corporate structure database (With G. Schmitt), design and maintenance of department website.